For Philip and Francesco :-)

Library preparation

Hi,
I hope, you find this useful.

My notes:

• it was part of exp. 20_Sequencing with Cristiana, I leave only my notes, no data; for that, you have to check my lab book …

Library Preparation for Illumina

• I find this one very usefull
• it includes also videos with “real life tips” on right side - description of each bellow

Library Preparation for Illumina: text & videos

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

INSTRUCTION MANUAL

Further resources

Pooling Calculator Illumina

Click

Setting up the PCR Reaction

Setting up the PCR Reaction - NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (E7600)

Multiplex Oligos for Illumina Protocol

Click

Learn about Illumina’s Next-Generation Sequencing Workflow

Illumina’s Next-Generation Sequencing Workflow