Sandwich for tranfer

Depend on available devices and can differ

Wet transfer - Biorad

• black piece (catode; negative)
• black sponge
• 2 x filter papers
• gel
• wet membrane in blotting buffer
• membrane
• 2 x filter papers
• black sponge
• white piece (anode; positive)

Semidry

• filter paper(s) (catode; negative)
• membrane
• gel
• filter paper(s) (catode; negative)